The collection of genes expressed, when they are expressed and how much they are expressed are what give a cell its personality. Consequently, regulating gene expression is very important and there are many molecular mechanisms through which it is achieved. Understanding these mechanisms provides a clearer picture to deciphering what goes wrong in cells when […]Read More Promoters as tumour suppressors: MYC vs. PVT1 for gene expression
We like DNA… a lot. It holds our genetic information; the heritable part of us that we receive from our forbearers and pass on to our young. But DNA is under attack. Every day a whopping 100,000 or so lesions form in our DNA in just one cell. This is not a problem, most of […]Read More GUARDIN the genome; a lncRNA unites p53, telomeres and miRNAs
Chromatin immunoprecipitation (ChIP) is basically a fancy term for a technique that allows you to see where a protein of interest binds DNA. It achieves this by crosslinking DNA and protein, chopping up the DNA and then extracting the DNA fragments that are attached to the protein you’re interested in using antibodies. Chen et al. […]Read More ChIPping transcription: how to map R-loops
2 meters doesn’t sound like much. However, this is the same length of DNA that is compacted inside the nucleus of each cell. Now, considering that nuclei have a diameter a fraction of a mm and nuclear proteins need to be included as well, this raises the important question, how does DNA fit? This apparent […]Read More Time to re-write the textbooks? How DNA is really packed inside the nucleus.
Biologists have a way of coming up with cool abbreviations; ChIP(Chromatin immunoprecipitation), DAPPER (DAtabase for Protein-Protein intERactions), ERGIC (ER–Golgi intermediate compartment) , TESCO (testis specific enhancer of Sox9 core element), but one of my personal favourites is CRISPR* – clustered regularly interspersed short palindromic repeats. *And as Hermione might say, it’s not crispaar, it’s, crisper. […]Read More CRISPR just got snappy…